vstispСадоводство и виноградарствоHorticulture and viticulture0235-25912618-9003Autonomous non-profit organization Editorial Board of journal «Horticulture and viticulture»10.31676/0235-2591-2023-5-35-41alcrtrvstisp-1088Research ArticleБИОТЕХНОЛОГИЯBIOTECHNOLOGYВлияние длительного депонирования клоновых подвоев яблони на питательных средах с добавлением жасмоновой кислоты и их последующее развитие в культуре in vitroLong-term cold storage of clonal apple rootstocks on growth media with added jasmonic acid: effect and their subsequent development in vitrohttps://orcid.org/0000-0001-7307-3217БъядовскийИ. А.BjadovskiyI. A.

Бъядовский Игорь Александрович – кандидат сельскохозяйственных наук, старший научный сотрудник, лаборатория репродуктивной биотехнологии

, ул. Загорьевская, 4, Москва, 115598

Bjadovskiy I. A., PhD (Agric.), Senior Researcher, Laboratory of Reproductive Biotechnology

4, Zagorevskaya str., Moscow, 115598, Russia

biakarachev@mail.ruФедеральный научный селекционно-технологический центр садоводства и питомниководстваРоссияFederal Horticultural Research Center for Breeding, Agrotechnology and NurseryRussian Federation202330112023053541Copyright © Autonomous non-profit organization Editorial Board of journal «Horticulture and viticulture», 20232023Autonomous non-profit organization Editorial Board of journal «Horticulture and viticulture»Autonomous non-profit organization Editorial Board of journal «Horticulture and viticulture»https://www.sadivin.com/jour/about/submissions#copyrightNoticehttps://www.sadivin.com/jour/article/view/1088

Изучено влияние жасмоновой кислоты в концентрациях от 0,1 до 1,0 мг/л при длительном беспересадочном депонировании эксплантов клоновых подвоев яблони (54-118, 57-490, 57-545, ММ 106) в культуре in vitro на их последующее развитие в стандартных условиях культивирования. Отмечено положительное влияние жасмоновой кислоты на сохранность жизнеспособных эксплантов (при +3…+6 ºС) по сравнению с эксплантами, которые культивировали без ее применения на протяжении 48-54 месяцев беспересадочного депонирования. Установлено, что разные концентрации растительного гормона существенно не влияли на коэффициент размножения эксплантов. При этом отмечено существенное увеличение длины микропобегов (в 1,4-1,6 раза) и среднего числа листьев на один микропобег (в 1,2-1,6 раза) у всех форм клоновых подвоев яблони на питательной среде с жасмоновой кислотой в концентрации 1,0 мг/л. В последующем экспланты не отставали в вегетативном развитии in vitro по сравнению с эксплантами, не проходившими депонирование (контроль). Добавление в питательную среду жасмоновой кислоты можно рекомендовать в качестве приема, повышающего сохранность эксплантов при длительном хранении коллекций клоновых подвоев яблони в культуре in vitro.

The article examines the effect of jasmonic a cid used at concentrations of 0.1-1.0 mg/L in the longterm continuous in vitro cold storage of clonal apple rootstock explants (54-118, 57-490, 57-545, and MM 106) on their subsequent development on under standard cultivation conditions. A positive effect of jasmonic acid was noted on the preservation of the viable explants of clonal apple rootstocks at all used concentrations over a period of 48-54 months (+3-6 ℃) of continuous cold storage, as compared to the explants cultivated without its application. Different concentrations of jasmonic acid in the cold storage medium were found to have no significant effect on the net reproduction of explants that were subsequently cultivated without jasmonic acid under standard cultivation conditions (+20-22 ℃). However, a significant increase in the length of microshoots (by 1.4-1.6 times) and the average number of leaves per microshoot (by 1.2-1.6 times) was observed in all studied forms of clonal apple rootstocks on growth medium with a jasmonic acid concentration of 1.0 mg/L. Subsequently, these explants exhibited no delay in vegetative development in vitro as compared to the explants that did not undergo cold storage (control). Jasmonic acid addition to the growth medium for long-term continuous cold storage at can be recommended as a way to improve the preservation of explants when clonal apple rootstock collections are stored for long periods of time in in vitro culture.

клональное микроразмножениеклоновый подвой яблонирегуляторы ростахранение коллекций in vitroразвитие эксплантов после депонированияclonal micropropagationclonal apple rootstockgrowth regulatorsin-vitro collection storagecold storageexplant developmentИсследования выполнены в рамках реализации государственного задания ФГБНУ ФНЦ Садоводства № 0432-2022-0001 «Воспроизводство и сохранение ценных генотипов плодовых и ягодных культур методами новых биотехнологий»The studies were carried out as part of implementing the state assignment No. 0432-2022-0001 of the Federal Horticultural Center for Breeding, Agrotechnology and Nursery “Reproduction and Conservation of Valuable Genotypes of Fruit and Berry Crops Using New Biotechnology Methods”ReferencesAsanidze Z., Akhalkatsi M., Henk A. D., Richards C. M., Volk G. M. Genetic relationships between wild progenitor pear (Pyrus L.) species and local cultivars native to Georgia, South Caucasus. Flora. 2014;209(9):504-512. 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The authors declare that there are no conflicts of interest present.