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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vstisp</journal-id><journal-title-group><journal-title xml:lang="ru">Садоводство и виноградарство</journal-title><trans-title-group xml:lang="en"><trans-title>Horticulture and viticulture</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0235-2591</issn><issn pub-type="epub">2618-9003</issn><publisher><publisher-name>Autonomous non-profit organization Editorial Board of journal «Horticulture and viticulture»</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.31676/0235-2591-2023-6-26-32</article-id><article-id custom-type="elpub" pub-id-type="custom">vstisp-1096</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ФИЗИОЛОГИЯ И БИОХИМИЯ РАСТЕНИЙ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PLANT PHYSIOLOGY AND BIOCHEMISTRY</subject></subj-group></article-categories><title-group><article-title>Подход к оценке засухоустойчивости винограда на модельных интактных листьях</article-title><trans-title-group xml:lang="en"><trans-title>Approach to assessing the drought resistance of grapes on model intact leaves</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1338-1725</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сундырева</surname><given-names>М. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Sundyreva</surname><given-names>M. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Сундырева М. А. − кандидат сельскохозяйственных наук, заведующий лабораторией физиологии и биохимии растений</p><p>ул. 40-летия Победы, д. 39, г. Краснодар, 350901</p></bio><bio xml:lang="en"><p>Sundyreva M. A., PhD (Agric.), Head of Laboratory of Plant Physiology and Biochemistry</p><p>39, 40-letiya Pobedy, Krasnodar, 350901</p></bio><email xlink:type="simple">mari.sundy@bk.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0521-0827</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Луцкий</surname><given-names>Е. О.</given-names></name><name name-style="western" xml:lang="en"><surname>Lutsky</surname><given-names>E. O.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Луцкий Е. О. − аспирант, младший научный сотрудник, лаборатория физиологии и биохимии растений</p><p>ул. 40-летия Победы, д. 39, г. Краснодар, 350901</p></bio><bio xml:lang="en"><p>Lutsky E. O., Postgraduate, Junior Researcher, Laboratory of Plant Physiology and Biochemistry</p><p>39, 40-letiya Pobedy, Krasnodar, 350901</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8425-5216</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мишко</surname><given-names>А. Е.</given-names></name><name name-style="western" xml:lang="en"><surname>Mishko</surname><given-names>A. E.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Мишко А. Е. − кандидат биологических наук, научный сотрудник, лаборатория физиологии и биохимии растений</p><p>ул. 40-летия Победы, д. 39, г. Краснодар, 350901</p></bio><bio xml:lang="en"><p>Mishko A. E., Ph.D. (Biol.), Researcher, Laboratory of Plant Physiology and Biochemistry</p><p>39, 40-letiya Pobedy, Krasnodar, 350901</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-4822-1139</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Схаляхо</surname><given-names>Т. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Shalyakho</surname><given-names>T. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Схаляхо Т. В. − младший научный сотрудник, лаборатория физиологии и биохимии растений</p><p>ул. 40-летия Победы, д. 39, г. Краснодар, 350901</p></bio><bio xml:lang="en"><p>Shalyakho T. V., Junior Researcher, Laboratory of Plant Physiology and Biochemistry</p><p>39, 40-letiya Pobedy, Krasnodar, 350901</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1152-5091</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Вялков</surname><given-names>В. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Vyalkov</surname><given-names>V. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Вялков В. В. − аспирант, младший научный сотрудник, лаборатория физиологии и биохимии растений</p><p>ул. 40-летия Победы, д. 39, г. Краснодар, 350901</p></bio><bio xml:lang="en"><p>Vyalkov V. V., Postgraduate, Junior Researcher, Laboratory of Plant Physiology and Biochemistry</p><p>39, 40-letiya Pobedy, Krasnodar, 350901</p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Северо-Кавказский федеральный научный центр садоводства, виноградарства, виноделия</institution><country>Россия</country></aff><aff xml:lang="en"><institution>North Caucasian Federal Scientifi c Center of Horticulture, Viticulture, Winemaking</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2023</year></pub-date><pub-date pub-type="epub"><day>20</day><month>12</month><year>2023</year></pub-date><volume>0</volume><issue>6</issue><fpage>26</fpage><lpage>32</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Autonomous non-profit organization Editorial Board of journal «Horticulture and viticulture», 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">Autonomous non-profit organization Editorial Board of journal «Horticulture and viticulture»</copyright-holder><copyright-holder xml:lang="en">Autonomous non-profit organization Editorial Board of journal «Horticulture and viticulture»</copyright-holder><license xlink:href="https://www.sadivin.com/jour/about/submissions#copyrightNotice" xlink:type="simple"><license-p>https://www.sadivin.com/jour/about/submissions#copyrightNotice</license-p></license></permissions><self-uri xlink:href="https://www.sadivin.com/jour/article/view/1096">https://www.sadivin.com/jour/article/view/1096</self-uri><abstract><p>Дефицит воды, с одной стороны, оказывает негативное воздействие на рост, развитие, долговечность и устойчивость винограда к болезням и низким температурам, но, с другой стороны, необходим для получения качественного урожая. В условиях усиления континентальности климата большое значение имеет поиск сортов, способных к качественному и эффективному плодоношению без ущерба виноградному растению в условиях засухи. Идентификация признака засухоустойчивости у винограда является сложной задачей ввиду размеров растений, длительного срока развития, отсутствия конкретного критерия засухоустойчивости, большой трудоёмкости и длительности поисковых исследований. В качестве модельной системы можно использовать интактные листья конкретного сорта винограда, помещённые в специфические внешние условия. Целью работы являлось изучение возможности использования интактных листьев винограда в качестве достоверной модели засухоустойчивости сорта. В работе проведено сравнение изменения физиологических параметров выхода электролитов, индекса стабильности хлорофилла и содержания малонового диальдегида в интактных листьях под воздействием сорбитола и в листовых дисках под воздействием полиэтиленгликоля различной концентрации и при различном времени экспозиции. В качестве эталона сравнения было использовано изменение физиологических параметров в листьях винограда, находящихся в условиях контролируемой почвенной засухи. Воздействие моделируемой интенсивной почвенной засухи в течение трёх недель в двух независимых экспериментах (2022-2023 гг.) выявило сортовые различия в соотношении выхода электролитов в «стрессовом» и контрольном вариантах, индексе стабильности хлорофилла. Аналогичные результаты были получены в эксперименте на интактных листьях винограда, помещенных в 10 %-й раствор сорбитола в течение 3 часов. На основании проведенного тестирования установлено, что сорта ‘Морозко’, ‘Кутузовский’ и ‘Достойный’ обладают более высокой засухоустойчивостью в сравнении с сортами ‘Каберне Совиньон’ и ‘Бархатный’. Данный подход может быть использован для предварительного определения засухоустойчивости сорта винограда как самостоятельно, так и в комплексе с полевыми исследованиями</p></abstract><trans-abstract xml:lang="en"><p>On the one hand, water scarcity poses challenges to the growth, development, longevity and resistance of grapes to diseases and low temperatures, on the other hand, however, it is necessary to obtain a high-quality harvest. Under the conditions of increasing continentality of the climate, it is of great importance to breed the cultivars characterised by effective fruiting without damaging the grape plant under drought conditions. Identifying characteristics of drought resistance in grapes is a challenging task, given the size of the plants, the extended development period, the absence of a specific criterion for drought resistance and the labour-intensive and time-consuming nature of the exploratory analysis. This study proposes the use of intact leaves from a specific grape variety placed in controlled environmental conditions as a model system. The paper assesses the applicability of intact grape leaves as a reliable model for evaluating drought resistance in the cultivar. It compares variations in physiological parameters—electrolyte leakage, chlorophyll stability index, and malonyldialdehyde content—observed in intact leaves under the influence of sorbitol and in leaf discs under the influence of polyethylene glycol with different concentrations and exposure times. The variations of physiological parameters in grape leaves subjected to controlled soil drought were used as a reference standard. In two independent experiments in 2022-2023, exposure to simulated intense soil drought for three weeks revealed cultivar differences in the ratio of electrolyte leakage and chlorophyll stability index in the test and control cultivars. Similar results were obtained in an experiment on intact grape leaves placed in a 10 % sorbitol solution for 3 hours. It was established that the cultivars ‘Morozko’, ‘Kutuzovsky’ and ‘Dostoiny’ exhibit higher drought resistance compared to the V. vinifera varieties ‘Cabernet Sauvignon’ and ‘Barkhatny’. This approach can be used as a preliminary method for determining the drought resistance of grape cultivars, independently or in combination with fi eld studies. </p></trans-abstract><kwd-group xml:lang="ru"><kwd>виноград</kwd><kwd>засуха</kwd><kwd>устойчивость</kwd><kwd>модельные условия</kwd><kwd>in vivo</kwd><kwd>физиологические параметры</kwd></kwd-group><kwd-group xml:lang="en"><kwd>grapes</kwd><kwd>drought</kwd><kwd>stability</kwd><kwd>model conditions</kwd><kwd>in vivo</kwd><kwd>physiological parameters</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена при финансовой поддержке гранта РНФ № 23-26-00280</funding-statement><funding-statement xml:lang="en">Th is work was supported by the grant of the RNF No. 23-26-00280</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Петров В. С., Алейникова Г. Ю., Новикова Л. 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