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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vstisp</journal-id><journal-title-group><journal-title xml:lang="ru">Садоводство и виноградарство</journal-title><trans-title-group xml:lang="en"><trans-title>Horticulture and viticulture</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0235-2591</issn><issn pub-type="epub">2618-9003</issn><publisher><publisher-name>Autonomous non-profit organization Editorial Board of journal «Horticulture and viticulture»</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.31676/0235-2591-2020-4-31-41</article-id><article-id custom-type="elpub" pub-id-type="custom">vstisp-555</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>СОВРЕМЕННЫЕ СИСТЕМЫ ВЕДЕНИЯ ПИТОМНИКОВОДСТВА</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>MODERN NURSERY</subject></subj-group></article-categories><title-group><article-title>Фундаментальные и практические основы создания полевого репозитория смородины чёрной в России</article-title><trans-title-group xml:lang="en"><trans-title>Fundamental and practical bases for creating a blackcurrant field repository in Russia</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1069-3771</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Упадышев</surname><given-names>М. Т.</given-names></name><name name-style="western" xml:lang="en"><surname>Upadyshev</surname><given-names>M. T.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Упадышев Михаил Тарьевич, доктор сельскохозяйственных наук, член-корреспондент РАН, главный научный сотрудник, заведующий лабораторией вирусологии, ул. Загорьевская, 4, Москва, 115598, Россия</p></bio><bio xml:lang="en"><p>Mikhail T. Upadyshev, Dr. Sci. (Agric.), Corresponding Member of the Russian Academy of Sciences, Senior Researcher, Head of Laboratory of Virology, 4, Zagoryevskaya st., Moscow, 115598, Russia</p></bio><email xlink:type="simple">virlabor@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8153-3009</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Метлицкая</surname><given-names>К. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Metlitskaya</surname><given-names>K. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>кандидат биологических наук, ведущий научный сотрудник, лаборатория вирусологии, Москва, Россия</p></bio><bio xml:lang="en"><p>PhD (Biol.), Leading Researcher, Laboratory of Virology, Moscow, Russia</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1760-5992</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сазонов</surname><given-names>Ф. Ф.</given-names></name><name name-style="western" xml:lang="en"><surname>Sazonov</surname><given-names>F. F.</given-names></name></name-alternatives><bio xml:lang="ru"><p>доктор сельскохозяйственных наук, ведущий научный сотрудник, отдел генетики и селекции плодовых и ягодных культур, Москва, Россия</p></bio><bio xml:lang="en"><p>Dr. Sci. (Agric.), Leading Researcher of the Department of Genetics and Breeding of Fruit and Small Fruit Crops, Moscow, Russia</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9256-0798</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Тумаева</surname><given-names>Т. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Tumaeva</surname><given-names>T. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>кандидат сельскохозяйственных наук, ведущий научный сотрудник, заместитель директора по научной работе, Москва, Россия</p></bio><bio xml:lang="en"><p>PhD (Agric.), Leading Researcher, Deputy Director for Research, Moscow, Russia</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4811-4280</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Петрова</surname><given-names>А. Д.</given-names></name><name name-style="western" xml:lang="en"><surname>Petrova</surname><given-names>A. D.</given-names></name></name-alternatives><bio xml:lang="ru"><p>кандидат сельскохозяйственных наук, старший научный сотрудник, лаборатория вирусологии, Москва, Россия</p></bio><bio xml:lang="en"><p>PhD (Agric.), Senior Researcher, Laboratory of Virology, Moscow, Russia</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7127-391X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Туть</surname><given-names>Е. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Tut’</surname><given-names>E. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>кандидат сельскохозяйственных наук, старший научный сотрудник, лаборатория вирусологии, Москва, Россия</p></bio><bio xml:lang="en"><p>PhD (Agric.), Senior Researcher, Laboratory of Virology, Moscow, Russia</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3849-8567</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Борисова</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Borisova</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>доктор сельскохозяйственных наук, профессор, главный научный сотрудник, отдел агротехнологий, Москва, Россия</p></bio><bio xml:lang="en"><p>Dr. Sci. (Agric.), Professor, Chief Researcher, Department of Agricultural, Moscow, Russia</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2676-9064</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Куликова</surname><given-names>О. З.</given-names></name><name name-style="western" xml:lang="en"><surname>Kulikova</surname><given-names>O. Z.</given-names></name></name-alternatives><bio xml:lang="ru"><p>агроном, отдел агротехнологий, Москва, Россия</p></bio><bio xml:lang="en"><p>Agronomist, Department of Agricultural Technologies, Moscow, Russia</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5820-102X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Радзениеце</surname><given-names>С. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Radzeniece</surname><given-names>S. B.</given-names></name></name-alternatives><bio xml:lang="ru"><p>младший научный сотрудник, лаборатория репродуктивной биотехнологии, Москва, Россия</p></bio><bio xml:lang="en"><p>Junior Researcher, Laboratory of Reproductive Biotechnology, Moscow, Russia</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1182-0867</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чердакли</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Cherdakli</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>младший научный сотрудник, лаборатория репродуктивной биотехнологии, Москва, Россия</p></bio><bio xml:lang="en"><p>Junior Researcher, Laboratory for Reproductive Biotechnology, Moscow, Russia</p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБНУ «Всероссийский селекционно-технологический институт садоводства и питомниководства»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>All-Russian Horticultural Institute for Breeding, Agrotechnology and Nursery</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2020</year></pub-date><pub-date pub-type="epub"><day>25</day><month>09</month><year>2020</year></pub-date><volume>0</volume><issue>4</issue><fpage>31</fpage><lpage>41</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Autonomous non-profit organization Editorial Board of journal «Horticulture and viticulture», 2020</copyright-statement><copyright-year>2020</copyright-year><copyright-holder xml:lang="ru">Autonomous non-profit organization Editorial Board of journal «Horticulture and viticulture»</copyright-holder><copyright-holder xml:lang="en">Autonomous non-profit organization Editorial Board of journal «Horticulture and viticulture»</copyright-holder><license xlink:href="https://www.sadivin.com/jour/about/submissions#copyrightNotice" xlink:type="simple"><license-p>https://www.sadivin.com/jour/about/submissions#copyrightNotice</license-p></license></permissions><self-uri xlink:href="https://www.sadivin.com/jour/article/view/555">https://www.sadivin.com/jour/article/view/555</self-uri><abstract><p>Создание свободного от вредоносных вирусов полевого репозитория смородины черной позволит провести оценку продуктивности, других качественных характеристик и использовать пыльцу здоровых растений для селекции. Целью исследований является выделение наиболее перспективных сортов смородины черной, тестирование ее на вирусы и получение исходных растений для закладки репозитория. Исследования проводили в 2018-2020 гг. с применением общепринятых методик сортоизучения, диагностики вирусов и размножения растений. Изучены ценные сорта смородины чёрной селекции Всероссийского селекционно-технологического института садоводства и питомниководства, отобраны и протестированы методами ИФА и ПЦР наиболее продуктивные клоны. Видовой состав вредоносных вирусов в плодоносящих насаждениях смородины черной в условиях ex situ разнообразен. Общая распространенность вирусов мозаики резухи (ArMV), кольцевой пятнистости малины (RpRSV), латентной кольцевой пятнистости земляники (SLRSV), черной кольцевой пятнистости томата (TBRV) на растениях смородины черной составила 32 %. Распространенность вирусов SLRSV и RpRSV около 15 %, TBRV – 14 %, ArMV – 7 %. Вирус реверсии смородины черной (BRV) отсутствовал. В результате проведенного мониторинга выделены свободные от вредоносных вирусов растения смородины черной 8 сортов, которые размножены без нарушения продуктивности и генетической стабильности и будут использованы для закладки репозитория и базисного маточника. </p></abstract><trans-abstract xml:lang="en"><p>Creating a virus-free repository of blackcurrant will allow an assessment of productivity, other qualitative characteristics and the use of pollen from healthy plants for breeding. The aim of the research was to reveal the most promising varieties and hybrids of blackcurrant, test plants for viruses and obtain nuclear stock plants for laying the repository. The studies were carried out in 2018-2020 using generally accepted methods of variety study, virus diagnosis and plants reproduction. Valuable blackcurrant varieties and hybrids of the All-Russian Horticultural Institute for Breeding, Agrotechnology and Nursery breeding were studied; the most productive clones were selected and tested by ELISA and PCR. The species composition of harmful viruses in the fruiting stands of blackcurrant under ex situ conditions is diverse. The total prevalence of Arabis mosaic virus (ArMV), Raspberry ringspot virus (RpRSV), Strawberry latent ringspot virus (SLRSV), Tomato black ring virus (TBRV) on blackcurrant plants was 31.5 %. The prevalence of SLRSV and RpRSV was about 15 %, TBRV – 14 %, ArMV – 7 %. Blackcurrant reversion virus (BRV) was absent. As a result of the monitoring carried out in 2018, blackcurrant plants of 8 varieties free from harmful viruses were identified and, after PCR, will be used to establish the repository and base mother nursery.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>смородина черная</kwd><kwd>кандидаты в исходные растения</kwd><kwd>сорта</kwd><kwd>репозиторий</kwd><kwd>вирусы</kwd><kwd>ИФА</kwd><kwd>ПЦР</kwd></kwd-group><kwd-group xml:lang="en"><kwd>black currant</kwd><kwd>candidates for nuclear stock plants</kwd><kwd>repository</kwd><kwd>viruses</kwd><kwd>ELISA</kwd><kwd>PCR</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Сазонов Ф. Ф. Селекция смородины чёрной в условиях юго-западной части Нечерноземной зоны России: монография. М.: ФГБНУ ВСТИСП, 2018, 304 с.</mixed-citation><mixed-citation xml:lang="en">Sazonov F. F. 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