Improving the efficacy of apple scab control using tank-mixes with microfertilizers over 2023–2024

While apple is the leading crop in Russian horticulture in terms of planted area and fruit production volume, high-quality yields are hindered by plant diseases that drastically reduce the market quality of fruit. The disease resistance of apple trees is largely determined by crop management practices. The most effective strategies include the systematic use of crop protection agents and the optimization of nutrient management. This approach enables a reduction in pathogen activity and an enhancement of natural defense mechanisms in apple trees. The present study aimed to investigate the effect of fungicides in tank-mixes with foliar fertilizers on the efficacy of apple orchard protection against apple scab, as well as on fruit yield and quality. The field trials were set up in the commercial plantings of the Dubovoye Company (Tambov Oblast) over 2023–2024. The evaluated cultivars included Bogatyr and Lobo. During the study period, weather conditions generally favored apple scab development, particularly in 2024. This can be attributed to spring frosts in early May following an early onset of vegetation growth and rapid plant development, subsequent cool weather, and heavy rainfall in June. The study evaluated apple scab development on leaves and fruits, biological efficacy, Zn and Mn concentrations in the leaves, and crop yield. Combining fungicides and microfertilizers within a single tank-mix reduced disease development and enhanced the biological efficacy of treatments. With such treatment, disease severity in Bogatyr trees ranged from 0.08 % to 0.7 %, while on the Lobo plot, it varied from 0.17 % to 2.5 %. The biological efficacy reached up to 96.7% for Bogatyr and 95.4 % for Lobo. Using tank-mixes of crop protection agents and foliar fertilizers secured fruit yields of 73.5 and 58.8 kg per tree, respectively, with the maximum proportion of fi rst-grade marketable fruit reaching 96 % and 92 %. The foliar fertilizer application contributed to an improved macroand micronutrient balance in the plants, which, in turn, enhanced the overall efficiency of fungicide treatments.

1. Kasatov I. S., Beloshapkina O. O. Biological eff ectiveness of systemic preparations against apple scab. Nauka i molodyozh’: aktual’nye voprosy i puti innovacionnogo razvitiya APK: materialy nac. nauch.-prakt. stud. konf., posvyashchennoj 45-letiyu FGBOU VO Belgorodskij GAU, p. Majskij, 06 iyunya 2023 g., Majskij: Belgorodskij GAU im. V. Ya. Gorina, 2023, 109-110. (in Russ.).

2. Nasonov A. I., Yakuba G. V. Apple scab: resistance to chemical fungicides, Mycology and Phytopathology. 2024;58(2): 91-107. DOI: 10.31857/S002636482. (in Russ.).

3. Kochkina A. M., Kashirskaya N. Ya. Biological effi ciency of protection systems for apple tree plantations against scab and apple moth, Achievements of Science and Technology of AIC, 2021;7:45-49. DOI: 10.53859/02352451_2021_35_7_45. (in Russ.).

4. Kazlouskaya Z. A., Hashenka T. A., Kandratsenak J. G., Martsinkevich T. M. Polymorphism of apple scab Venturia inaequalis in Belarus, Molekulyarnaya i prikladnaya genetika. 2021;30:68-76. DOI: 10.47612/1999-9127-2021-30-68-76. (in Russ.).

5. Yakuba G. V. Adaptive changes in the apple scab pathogen under weather stress conditions, Plant Protection and Quarantine. 2014;4:26-29. (in Russ.).

6. Komardina V. S., Pleskatsevich R. I., Vasekha E. V. Efficiency of the fungicide Zato plus, WDG in the system of apple-tree protection against the diseases, Plant Protection. 2021;(45):137-144. DOI: 10.47612/0135-3705-2021-45-137-144. (in Russ.).

7. Komardina V. S., Vasekha E. V., Pleskatsevich R. I. Sensitivity of the causal agent of apple scab Venturia inaequalis fungus to kresoxim-methyl in commercial orchards of Belarus, Plant Protection. 2022; 1(46):103-110. DOI: 10.47612/0135-3705-202246-103-110. (in Russ.).

8. Shapovalov M. Yu., Khilevsky V. A. Long-term monitoring of Venturia inaequalis (apple scab): Plant Protection against Harmful Organisms. Mat. IX mezhd. nauch.-praktyu konf., Krasnodar, 17-19 iyunya 2019, 317-318. (in Russ.).

9. Kuksenko S. G., Dubin R. I. Apple tree protection system from scab in the Astrakhan region, Estestvennye nauki. 2023;4(13):69-76. DOI: 10.54398/1818507X_2023_4_69. (in Russ.).

10. Csihon Á., Gonda I., Sipos M., Holb I. J. Impacts of N-P-K-Mg Fertilizer Combinations on Tree Parameters and Fungal Disease Incidences in Apple Cultivars with Varying Disease Susceptibility, Plants. 2024;13(9):1217. DOI: 10.3390/plants13091217.

11. Tripathi R., Tewari R., Singh K. P., Keswani C., Minkina T., Srivastava A. K., De Corato U., Sansinenea E. Plant mineral nutrition and disease resistance: A signifi cant linkage for sustainable crop protection. Frontiers in Plant Science. 2022;13:883970. DOI: 10.3389/fpls.2022.883970.

12. Sazanova K.V., Zelenskaya M. S., Korneev A.V., Vlasov D. Yu. Extracellular zinc detoxifi cation by the fungi Penicillium chrysogenum and Aspergillus niger , Mycology and phytopathology. 2023;57(6): 425-434. DOI: 10.31857/S0026364823060089. (in Russ.).

13. Yadav A., Sohlot M., Sahu S. R., Banerjee T., Bhattacharya J., Bandyopadhyay K., Das S., Debnath N. Determination of antifungal effi cacy and phytotoxicity of a unique silica coated porous zinc oxide nanocomposit e medium for slow-release agrochemicals, Journal of Applied Microbiology. 2024;135(7):lxae153. DOI: 10.1093/jambio/lxae153.

14. Vijayreddy D., Kandan A., Dutta P., Pathak, J. Dual role of zinc oxide nanoparticles in antifungal action against Rhizoctonia solani , biosafety and phytotoxicity concerns in crop plants, Physiological and Molecular Plant Pathology. 2025;138:102712. DOI: 10.1016/j.pmpp.2025.102712.

15. Reuveni R., Reuveni M. Foliar-fertilizer therapy a concept in integrated pest management, Crop Protection. 1998;17(2):111-118. DOI: 10.1016/S0261-2194(97)00108-7.

16. Shahrajabian M. H., Sun W., Cheng Q. Foliar application of nutrients on medicinal and aromatic plants, the sustainable approaches for higher and better production, Beni-Suef University Journal Basic and Applied Science. 2022;11:26. DOI: 10.1186/s43088-022-00210-6.

17. Podgornaya M.E., Yakuba G.V., Mishchenko I.G., Didenko N.A., Yakuba Yu.F. The infl uence of triazole fungicides on agrobiological, biochemical, and toxicological parameters of apple and plum trees, Nauchnye trudy SKFNCSVV. 2020;28:62-68. (in Russ.). DOI: 10.30679/2587-9847-2020-28-62-68. (in Russ.).

18. Yakuba G. V., Astapchuk I. L., Nasonov A. I. In vitro action of fungicides on fungi of the genus fusarium link causing core rot of apple-fruit, Taurida herald of the agrarian sciences. 2020;2(22):188-197. DOI: 10.33952/2542-0720-2020-2-22188-197. (in Russ.).

19. Kuzin A. I., Kashirskaya N. Ya., Kochkina A. M. Elaboration of an ecologized scab protection system and a biologized apple plant nutritional program, Sadovodstvo i vinogradarstvo. 2020;4:42-48. DOI: 10.31676/0235-2591-2020-4-42-48. (in Russ.).

20. Kashirskaya N. Ya., Kuzin A. I., Kochkina A. M., Sviridov G. B., Nazarov V. N. Eff ect of the combined application of plant protection products and foliar fertilizers on the development of apple scab, fruit quality, and productivity, Sadovodstvo i vinogradarstvo. 2024;3:41-48. DOI: 10.31676/0235-2591-2024-3-41-48. (in Russ.).

21. Kasatov I. S., Beloshapkina O. O., Khokhlov A. A. Apple scab development: an agro-climatic and phytopathological study in a Moscow Region orchard, Izvestiya of Timiryazev Agricultural Academy. 2025;3:96-114. DOI: 10.26897/0021-342X-2025-396-114. (in Russ.).

22. Antonenko V. V., Zubkov A. V., Kruchina S. N. Peculiarities of the development of apple trees and pear fungous diseases under the conditions of Non-black Earth Zone, Bulletin of Agrarian Science. 2020;2(83):9-14. DOI: 10.17238/issn2587-666X.2020.2.9. (in Russ.).

23. Methodological Guidelines for Registration Trials of Fungicides in Agriculture. Pod red. V. I. Dolzhenko. Sankt-Peterburg, 2009, 378 s. (in Russ.).

24. Dospekhov B. A. Methods of Field Experimentation (with Fundamentals of Statistical Processing of Research Results). M.: Agropromizdat, 1985, 351 s. (in Russ.).

25. Mineev V. G., Sychev V. G., Amelyanchik O. A., Bolysheva T. N., Gomonova N. F., Durynina E. P., Egorov B. S., Egorova E. V., Edemskaya N. L., Karpova E. A., Mezhukova V. G. Workshop on agrochemistry. M.: Izd-vo MGU, 2001. 689 c. (in Russ.).

26. Tserling V. V. Diagnostics of Crop Nutrition. M.: Agropromizdat, 1990, 235 s. (in Russ.).

27. Artsybasheva N. S., Yushkov A. N., and Kuzin A. I. The Effect of Macro and Microelement Treatments on the Winter Hardiness of Promising Apple Varieties: Scientifi c Achievements for the Eff ective Development of Horticulture, Mat. nauch.-prakt. konf. (5 sentyabrya, Michurinsk, 2025 g., Michurinsk-naukograd RF, 2025, 12-17. (in Russ.).

28. Lim-Hing S., Gandhi K. J. K., Villari C. The role of Manganese in tree defenses against pests and pathogens, Plant Physiology and Biochemistry. 2024;210:108641. DOI: 10.1016/j.plaphy.2024.108641.

29. Thiébaut N., Hanikenne M. Zinc deficiency responses: bridging the gap between Arabidopsis and dicotyledonous crops, Journal of Experimental Botany. 2022;73(6):1699-1716. DOI: 10.1093/jxb/erab491.

30. Pacini T., Orsini S., Verdini E., Cristofani E., Pelliccia A., Sdogati S., Colosio C. Pecorelli I. Advances in the Analytical Determination and Toxicological Assessment of Dithiocarbamates and Their Hydrolysis Products in Fruits, Vegetables, and Cereals: Methodological Evolution, Challenges, and Future Directions, Toxics. 2025;13(10): 819. DOI: 10.3390/toxics13100819.

31. Grischechkina L. D., Dolzhenko V. I., Milyutenkova T. I. Modern fungicides for orchard protection, Plodovodstvo i yagodovodstvo Rossii. 2012;30:408-422. (in Russ.).

32. Khilevsky V.A. Fungicides to protect apple-tree from phytopathogens complex. Vestnik ORELGAU. 2015;5(56):40-45. DOI: 10.15217/issn1990-3618.2015.5.39. (in Russ.).

33. Yakuba G. V. Apple technologies protection from diseases with domestic fungicides application, Plant Protection. 2016;4:33-39. DOI: 10.18454/VSTISP.2016.4.2841. (in Russ.).