Structural and functional changes in the leaves of black currant seedlings using nanoparticle solutions

When propagating black currant by lignified cuttings in order to obtain high-quality planting material in one growing season in the conditions of the forest-steppe zone of the Krasnoyarsk Territory, solutions of biogenic ferrihydrite nanoparticles were used in three modifications with the addition of traditional growth hormone (indolyl-3-acetic acid): control (soaking cuttings in water); treatment of cuttings with indolyl-3-acetic acid (IAA); IAA+ferrihydrite (IAA+Feh); IAA+Co-doped ferrihydrite (IAA+Feh_Co); IAA+ferrihydrite doped with Mn (IAA+Feh_Mn). The object of the study is blackcurrant plants of the Selechenskaya variety. The area of the assimilation surface and the pigment composition of the leaves were assessed in dynamics. Before planting, black currant cuttings were soaked in nanoparticle solutions (exposure 12 h), then, during the growing season, four times (with an interval of 14 days) exogenous treatment of the leaf surface of rooted cuttings with the same solutions of nanoparticles, but without the addition of IAA, was carried out. The results of studying the concentration of pigments in leaves and biometric observations in the dynamics of the growing seasons of 2021 and 2022 are presented. It has been established that the maximum concentration of chlorophyll a and b, carotenoids in black currant leaves by the end of the growing season was affected by the use of solutions of ferrihydrite nanoparticles doped with cobalt and manganese with the addition of IAA. Thus, the concentration of chlorophyll a, b increased by 1.3 and 1.6 times, respectively, in the variant with cobalt-doped ferrihydrite nanoparticles compared to the control. In our studies, the values of the assimilation surface of leaves depended on the climatic parameters of the year of study and on the content of photosynthetic pigments in blackcurrant leaves. Between the area of the leaf blade and the concentration of chlorophyll a, a direct relationship was noted (r=0,87), a close relationship: in 2021 between the area of the leaf blade of black currant and the amount of precipitation (r=0,83-0.94), in 2022 between the area of blackcurrant leaf blade and air temperature (r=0,85-0,93). It has been established that the best conditions for the formation of the assimilation surface of blackcurrant leaves of the Selechenskaya variety are created by soaking cuttings and foliar treatment of vegetative plants with ferrihydrite nanoparticles doped with cobalt and manganese.

1. Bopp V. L., Kuz’mina Ye. M., Mistratova N. A. Plodovodstvo Sibiri. Krasnoyar. gos. agrar. un-t., Krasnoyarsk, 2020, 387 р. (In Russ)

2. Mistratova N. A., Bopp V. L. Vliyaniye sootnosheniya kory k ksileme na okoreneniye zelenykh cherenkov kryzhovnika (Grossularia Mill). Sadovodstvo i vinogradarstvo. 2017;1:39-42. (In Russ)

3. Kuprina M. N., Kolesnikova V. L. Ispol’zovaniye stimulyatorov rosta v yagodnom pitomnike. Vestnik KrasGAU. 2014;7(94):85-91. (In Russ)

4. Bopp V. L., Kuprina M. N. Nauchnyye osnovy razmnozheniya smorodiny krasnoy i oblepikhi odrevesnevshimi cherenkami v usloviyakh lesostepi Krasnoyarskogo kraya; Krasnoyar. gos. agrar. un-t. Krasnoyarsk, 2018, 168 p. (In Russ)

5. Getko N. V. Rasteniya v tekhnogennoy srede: Struktura i funktsiya assimilyatsionnogo apparata. Monografi ya. Minsk, Nauka i tekhnika, 1989, 205 р. (In Russ)

6. Zborovskaya O. V., Pryadkina G. A., Oksem V. P. Zavisimost’ khlorofil’nogo indeksa posevov vysokoproduktivnykh sortov ozimoy pshenitsy ot usloviy vyrashchivaniya i yego svyaz’ s produktivnost’yu. Zemledeliye i selektsiya v Belorusi. 2016;52:88-95. (In Russ)

7. Lankina Ye. P., Shevelev D. I., Khizhnyak S. V. i dr. Issledovaniye antitoksicheskikh svoystv biogennykh nanochastits gidroksida zheleza v otnoshenii tiabendazol-tebukonazolovykh fungitsidov. Vestnik KrasGAU. 2011;11:129-133. (In Russ)

8. Tarasova Ye. YU., Korosteleva V. P., Ponomarev V. YA. Primeneniye nanotekhnologiy v sel’skom khozyaystve. Vestnik Kazanskogo tekhnologicheskogo universiteta. 2012;4:121-122. (In Russ)

9. Yurin V. M., Molchan O. V. Nanomaterialy i rasteniya: vzglyad na problemu: Trudy Belorusskogo gosudarstvennogo universiteta. Seriya: Fiziologicheskiye, biokhimicheskiye i molekulyarnyye osnovy funktsionirovaniya biosistem. 2015;10(1):9-21. (In Russ)

10. Suchkova S. A., Astafurova T. P. Morfologicheskiye izmeneniya v cherenkakh smorodiny chernoy pod vliyaniyem nanochastits oksida tsinka: Rol’ fi ziologii i biokhimii v introduktsii i selektsii ovoshchnykh, plodovo-yagodnykh i lekarstvennykh rasteniy: mater. III mezhdun. konf.. M., RUDN, 2017, 312-315. (In Russ)

11. Mistratova N. A., Gurevich YU. L., Teremova M. I., Kolesnik A. A. Opyt ispol’zovaniya nanochastits gidroksida zheleza pri razmnozhenii Ribes nigrum L. zelenymi cherenkami. Vestnik KrasGAU. 2019;11:16-23. (In Russ)

12. Suchkova S. A., Yamburov M. S., Astafurova T. P., Sirotkina E. E. Iron oxyhydroxide eff ect on rooting of cuttings of Ribes nigrum and Ribes rubrum. International Journal of GEOMATE. 2019;17.61:169-173. DOI: 10.21660/2019.61.17816.

13. Shlyk A. A. O spektrofotometricheskom opredeleniye khlorofi llov a i b. Biokhimiya. 1968;33:(2):275-285. (In Russ)

14. Dospekhov V. A. Metodika polevogo opyta. M.: Agroproizdat, 1985, 351 р. (In Russ).

15. Korotchenko I. S. Vliyaniye tyazhelykh metallov na soderzhaniye fotosinteticheskikh pigmentov v list’yakh morkovi. Vestnik KrasGAU. 2011;4(55):86-91. (In Russ)

16. Medvedeva V. A., Korotchenko I. S. Otsenka vozmozhnosti primeneniya nuta dlya ochistki sredy ot tyazhelykh metallov. Vestnik KrasGAU. 2020;10(163):8894. (In Russ)

17. Lebedev S. V. Otsenka vliyaniya nanochastits zheleza FeO, nanochastits magnetita Fe3O4 i sul’fata zheleza (II) FeSO4 na soderzhaniye fotosinteticheskikh pigmentov Triticum vulgare. Fiziologiya rasteniy. 2014;61(4):603. DOI: 10.7868/S0015330314040125. (In Russ)

18. Astafurova T. P., Morgalev YU. N., Zotikova A. P. i dr. Vliyaniye nanochastits dioksida titana i oksida alyuminiya na morfofi ziologicheskiye pokazateli rasteniy. Vestn. Tomsk.gos. un-ta. Biologiya. 2011;1(13):113-122. (In Russ)

19. Gur’yanova YU. V., Ryazanova V. V. Formirovaniye ploshchadi list’yev i soderzhaniye khlorofi lla v list’yakh pri meneral’nom pitanii. Vestnik MichGAU. 2012;4:30-32. (In Russ)