Nutritional value of fruits of different highbush blueberry cultivars

Highbush blueberry is one of the most common berry crops. The presence of polyphenolic compounds, especially anthocyanins and proanthocyanidins, determines the high nutritional value of blueberry fruits, as well as their potential health benefits for humans. A large number of studies have shown that regular highbush blueberry consumption can reduce the development and progression risk of several diseases caused by inflammation and oxidative stress. Moreover, it can positively affect the composition of the human intestinal microbiome. The work aims to study the content and composition of the main groups of polyphenolic compounds, organic acids, and sugars in fresh fruits of 7 blueberry cultivars (Coville, Elliott, Bluray, Bluegold, Goldtraube 71, Spartan, Rancocas). Fruits were collected from plants grown at the experimental cultivar testing site of the I.V. Michurin Federal Research Center. They were examined using modern analytical methods such as spectrophotometry, HPLC with photo-, refracto-, and mass-spectrometric detection. The content and profile of anthocyanins, proanthocyanidins, catechins, flavonols, hydroxycinnamic acids, organic acids, and sugars were examined in detail. In addition, the study determined the main specific chemotaxonomic features in the cultivar context. Drawing on the obtained results, cultivars with the highest anthocyanin and proanthocyanidin contents were identified. The article shows that the studied cultivars have potential for being used as raw materials to increase the nutritional value of human diets.

1. Golovinskaia O., Wang C. K. Review of Functional and Pharmacological Activities of Berries, Molecules. 2021;26(13):3904. DOI: 10.3390/molecules26133904.

2. Del Bo’ C., Tucci M., Martini D., Marino M., Bertoli S. (et al.) Acute eff ect of blueberry intake on vascular function in older subjects: Study protocol for a randomized, controlled, crossover trial, PLoS One. 2022;17(12):1-16. DOI: 10.1371/journal.pone.0275132.

3. Curtis P., Berends L., van der Velpen V. (et al.) Blueberry anthocyanin intake attenuates the postprandial cardiometabolic eff ect of an energy-dense food challenge: Results from a double blind, randomized controlled trial in metabolic syndrome participants, Clinica Nutrition. 2022;41(1):165-176. DOI: 10.1016/j.clnu.2021.11.030.

4. Sun W. A review of the physiological functions of blueberry anthocyanins and their applications in food, BIO Web of Conferences. 2023;59:02011. DOI: 10.1051/bioconf/20235902011.

5. Onuh J. O., Dawkins N. L., Aluko R. E. Cardiovascular disease protective properties of blueberry polyphenols (Vaccinium corymbosum): a concise review, Food Prod, Process and Nutr. 2023;5:27. DOI: 10.1186/s43014-023-00139-y.

6. Wu Y., Han T., Yang H., Lyu L., Li W., Wu W. Known and potential health benefi ts and mechanisms of blueberry anthocyanins: A review, Food Bioscience. 2023;55:103050. DOI: 10.1016/j.fbio.2023.103050.

7. Wang L., Lan W., Chen D. Blueberry (Vaccinium spp.) Anthocyanins and Their Functions, Stability, Bioavailability, and Applications, Foods. 2024;13(17):2851. DOI: 10.3390/foods13172851.

8. Tutel’yana V. A, Ellera K. I. Methods of analysis of minor biologically active substances of food Ed. V. A. Tutellan, K. I. Eller. Moscow: Dynasty; 2010, 28-41. (in Russ.).

9. Perova I. B., Eller K. I., Gerasimov M. A., Baturina V. A., Akimov M. Yu., Akimova O. M., Mironov A. M., Koltsov V. A. A study of a complex of bioactive compounds in the fruits of promising blue honeysuckle (Lonicera caerulea L.) cultivars, Proceedings on Applied Botany, Genetics and Breeding. 2023;184(1):53-69. DOI: 10.30901/2227-8834-2023-1-1-17. (in Russ.).

10. Gerasimov M. A., Perova I. B., Eller K. I., Akimov M. Y., Sukhanova A. M., Rodionova G. M., Ramenskaya G. V. Investigation of Polyphenolic Compounds in Diff erent Varieties of Black Chokeberry Aronia melanocarpa, Molecules. 2023;28(10):4101. DOI: 10.3390/molecules28104101.

11. Wang S., Wang B., Dong K., Li J., Li Y., Sun H. Identifi cation and quantifi cation of anthocyanins of 62 blueberry cultivars via UPLC-MS, Biotechnology & Biotechnological Equipment. 2022;36(1):587-597. DOI: 10.1080/13102818.2022.2090857.

12. Wang Y., Fong S. K., Singh A. P., Vorsa N., Johnson-Cicalese J. Variation of Anthocyanins, Proanthocyanidins, Flavonols, and Organic Acids in Cultivated and Wild Diploid Blueberry Species, HortScience horts. 2019;54(3):576-585. DOI: 10.21273/HORTSCI13491-18.

13. Lachowicz-Wiśniewska S., Pratap-Singh A., Ochmian I., Kapusta I., Kotowska A., Pluta S. Biodiversity in nutrients and biological activities of 14 highbush blueberry (Vaccinium corymbosum L.) cultivars. Sci Rep. 2024.27;14(1):22063. DOI: 10.1038/s41598-024-71114-x.

14. Prior R. L., Lazarus S. A., Cao G., Muccitelli H., Hammerstone J. F. Identifi cation of Procyanidins and Anthocyanins in Blueberries and Cranberries (Vaccinium Spp.) Using High-Performance Liquid Chromatography/Mass Spectrometry, Journal of Agricultural and Food Chemistry. 2001;49(3):1270-1276. DOI: 10.1021/jf001211q.

15. Rossi G., Woods F. M., Leisner C. P. Quantifi cation of Total Phenolic, Anthocyanin, and Flavonoid Content in a Diverse Panel of Blueberry Cultivars and Ecotypes, HortScience. 2022;57(8):901-909. DOI: 10.21273/HORTSCI16647-22.

16. Giovanelli G., Buratti S. Comparison of Polyphenolic Composition and Antioxidant Activity of Wild Italian Blueberries and Some Cultivated Varieties, Food Chemistry. 2009;112(4):903- 908. DOI: 10.1016/j.foodchem.2008.06.066.

17. Bunea A., Rugină D., Pintea A., Diaconeasa Z., Bunea C.I., Socaciu C. Comparative Polyphenolic Content and Antioxidant Activities of Some Wild and Cultivated Blueberries from Romania, Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2011;39(2):70-76. DOI: 10.15835/nbha3926265.

18. Kim J. G., Kim H. L., Kim S. J., Park K. S. Fruit quality, anthocyanin and total phenolic contents, and antioxidant activities of 45 blueberry cultivars grown in Suwon, Korea, J Zhejiang Univ Sci B. 2013;14(9):793-799. DOI: 10.1631/jzus.B1300012.

19. Li D., Li B., Ma Y., Sun X., Lin Y., Meng X. Polyphenols, anthocyanins, and fl avonoids contents and the antioxidant capacity of various cultivars of highbush and half-high blueberries, Journal of Food Composition and Analysis. 2017;62:84-93. DOI: 10.1016/j.jfca.2017.03.006.

20. Zorenč Z., Veberic R., Stampar F., Koron D., Mikulic-Petkovsek M. Changes in berry quality of northern highbush blueberry (Vaccinium corymbosum L.) during the harvest season, Turkish journal of agriculture and forestry. 2016;40:855-864. DOI: 10.3906/tar-1607-57.

21. Pico J., Yan Y., Gerbrandt E., Castellarin S. D. Determination of free and bound phenolics in northern highbush blueberries by a validated HPLC/QTOF methodology, Journal of Food Composition and Analysis. 2022;108:104412. DOI: 10.1016/j.jfca.2022.104412.

22. Zhang J., Nie J.-Y., LI J., Zhang H., LI Y., Farooq S., Bacha S.A.S., Wang J. Evaluation of sugar and organic acid composition and their levels in highbush blueberries from two regions of China, Journal of Integrative Agriculture. 2020;19(9):2352-2361. DOI: 10.1016/S2095-3119(20)63236-1.

23. Xu Y., LI K., Wang H., Chen L., Gong X. Determination of Eight Organic Acids in Blueberries by HPLC, Food Science. 2015;26(18):127-131. DOI: 10.7506/spkx1002-6630-201518023.

24. Smrke T., Veberic R., Hudina M., Stamic D., Jakopic J. Comparison of Highbush Blueberry (Vaccinium corymbosum L.) under Ridge and Pot Production, Agriculture. 2021;11(10):929. DOI: 10.3390/agriculture11100929.

25. Šne E., Kampuse S., Berņa E. The composition of sugars and sugar-acid ratio of highbush blueberry varieties grown in Latvia, Research for Rural Development - International Scientifi c Conference. 2011;1:140-144.

26. Akšić M.F., Tosti T., Sredojević M., Milivojević J., Meland M., Natić M. Comparison of Sugar Profi le between Leaves and Fruits of Blueberry and Strawberry Cultivars Grown in Organic and Integrated Production System, Plants (Basel). 2019;8(7):205. DOI: 10.3390/plants8070205.