Screening methods to identify the complex of strains of Plum pox virus (PPV)
https://doi.org/10.31676/0235-2591-2019-1-36-42
Abstract
From the 10 known strains of thePlumpox virus (PPV) in the Russian Federation 7 strains are spread – D, M. Rec, W, C, CV, and CR with the prevalence of D and W strains. A characteristic feature of the Plum pox virus population in the Russian Federation is the wide spread of W, C and CR strains, extremely rare or not found at all in other regions of the world. Screening tests for detection of the complex of PPV strains by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) were carried out. It has been established that effective detection of PPV-D, PPV-M, PPV-Rec, PPV-C, PPV-CR, PPV-EA, PPV-W and PPV-T strains is achieved using PPV-ELISA test systems with polyclonal antibodies from ACD, Adgen, Agdia, Bioreba, DSMZ and Loewe companies. Monoclonal antibodies 5B-IVIA do not allow to diagnose isolates related to the strain PPV-CR. For universal detection of isolates of all PPV strains, it is necessary to use a real-time PCR kit for PPV (Syntol), a FLASH-PCR kit for PPV (Agrodiagnostica) or conventional PCR with one of the following primer pairs: P1/P2, s1/as2 or 3´NCP sense/3´NCP antisense.
About the Authors
Yu. N. Prikhodko
All-Russian Plant Quarantine Centre
Russian Federation
Russian Federation
PhD (Agric.), Leader Researcher
Address for correspondence: Prikhodko Yuriy N. – All-Russian Plant Quarantine Center, 140150, Russia, Moscow region, Ramenskiy district, Bykovo-2, Pogranichnaya str., 32
T. S. Zhivaeva
All-Russian Plant Quarantine Centre
Russian Federation
Reseacher
Russian Federation
Reseacher
Yu. A. Shneyder
All-Russian Plant Quarantine Centre
Russian Federation
Head of Department, PhD (Biol.)
Russian Federation
Head of Department, PhD (Biol.)
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Review
For citations:
Prikhodko Yu.N., Zhivaeva T.S., Shneyder Yu.A. Screening methods to identify the complex of strains of Plum pox virus (PPV). Horticulture and viticulture. 2019;(1):36-42. (In Russ.) https://doi.org/10.31676/0235-2591-2019-1-36-42
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