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Polymorphism study of raspberry (Rubus idaeus L.) cultivars and hybrids using ISSR-PCR method: identifying forms potentially resistant to Raspberry bushy dwarf virus
https://doi.org/10.31676/0235-2591-2023-6-10-17
Abstract
The article presents the results of DNA testing for 21 raspberry cultivars using the ISSR-PCR method to study genetic polymorphism and identify genotypes potentially resistant to the raspberry bushy dwarfvirus (RBDV). It was revealed that promising hybrids of everbearing raspberry (Rubus idaeus L.), including 9-155-1, 11-165-10 and 9-163-1, exhibited significant genetic relations with RBDV-resistant cultivars. Hybrids 9-163-1 and 11-165-10, both having the hybrid 13-118-1 as one of the parent forms, were grouped with the RBDV-resistant cultivar ‘Poklon Kazakovu’. These forms showed similarities with the resistant cultivars ‘Meteor’ and ‘Atlant’, as well as the sensitive cultivars ‘Evrasia’ and ‘Podarok Kashinu’. The hybrid 9-155-1 was characterized by genetic proximity with the sensitive cultivars ‘Podarok Kashinu’ and ‘Evrasia’, as well as the resistant cultivars ‘Meteor’, ‘Atlant’, ‘Zhar-Ptitsa’, and ‘Heritage’. The Heritage variety is known to be resistant to RBDV, attributed to the Bu gene. This gene is responsible for the resistance to the virus, including the most dangerous isolate R15. The analysis of the constructed dendrites suggested the potential resistance to RBDV in the hybrid 9-155-1.
About the Authors
E. V. Tarasova
Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery
Russian Federation
Russian Federation
Tarasova E. V., Junior Researcher, Laboratory of Reproductive Biotechnology
4, Zagorevskaya str., Moscow, 115598
I. A. Кapitova
Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery
Russian Federation
Russian Federation
Kapitova I. A., PhD (Chem.), Senior Researcher, Head of Laboratory of Reproductive Biotechnology
4, Zagorevskaya str., Moscow, 115598
S. N. Evdokimenko
Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery
Russian Federation
Russian Federation
Evdokimenko S. N., Dr. Sci. (Agric.), Chief Researcher
4, Zagorevskaya str., Moscow, 115598
E. V. Nemtsova
Bryansk State University named aft er Academician I. G. Petrovsky
Russian Federation
Russian Federation
Nemtsova E. V., PhD (Biol.), Docent
Bryansk
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Review
For citations:
Tarasova E.V., Кapitova I.A., Evdokimenko S.N., Nemtsova E.V. Polymorphism study of raspberry (Rubus idaeus L.) cultivars and hybrids using ISSR-PCR method: identifying forms potentially resistant to Raspberry bushy dwarf virus. Horticulture and viticulture. 2023;(6):10-17. (In Russ.) https://doi.org/10.31676/0235-2591-2023-6-10-17
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