Patterns of endophytic bacteria prevalence in flowers of the Debryansk black currant cultivar and its parental forms

   Microbiological analysis on flower samples of the Debryansk black currant cultivar and its parental forms – the Lentyay maternal cultivar (♀) and the Yadrenaya paternal cultivar (♂) – was conducted. Samples were collected from plantings of the genetic collection from the Shared Facility Center Genetic Bioresource Collection of Plants of the Federal Scientific Center of Horticulture (Kokino Outpost, Bryansk Oblast). Cultivable endophytic bacteria (EB) were isolated from the flowers on both rich and nutrient-poor solid media, including MPA, TSA, CAA, Ashby, Potato Agar, and LB Agar. Based on cultural and morphological characteristics, several distinct taxa of cultivable EB were identifi ed in the fl owers. The Debryansk cultivar generally exhibited a higher abundance of EB colonies with diverse cultural and morphological features compared to its parental forms. Among the parental cultivars, Lentyay demonstrated a higher taxonomic richness of EB on both rich (up to 8,375,200 CFU/g on TSA) and poor media (up to 1,265,400 CFU/g on LB Agar). The lower ratios of EB abundance in the flowers of the Yadrenaya paternal cultivar to that in Debryansk suggest a more significant contribution of the maternal line in shaping the microbiome of black currant cultivar. Correlation analysis indicates that the breeding process facilitated the harmless transmission of the ability to accumulate specific bacterial communities in internal tissues from the parental forms to Debryansk. The Lentyay maternal cultivar and the Yadrenaya paternal cultivar contributed saprophytic forms utilizing organic nitrogen compounds and heterotrophic forms assimilating inorganic nitrogen compounds. Additionally, the Yadrenaya paternal cultivar contributed aerobic nitrogen-fixing bacteria.

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