Peculiarities of clonal micropropagation of red raspberry (Rubus idaeus L.) of Altai breeding

Abstract

The article presents the results of research on optimization of clonal micropropagation of raspberry (Rubus idaeus L.). The aim of research was to study the infl uence of growth regulators and components of nutrient medium at micropropagation in vitro of raspberry new varieties Aquarel` and Dobraya which were breeded in the Altai region. These varieties have characteristics such as: high winter hardiness, thorn free, resistance to diseases, large, aromatic and sweet berries. The effect of infl uence of growth regulators: 6-benzylaminopurine (BAP), tidiazuron (TDZ), zeatin, indolyl butyric acid (IBA) and elements of mineral nutrition - nitrogen, ferrum and carbohydrates in nutrient medium on the multiplication of new varieties of raspberry has been studied. It was found, that BAP had the best inducing effect at micropropagation. With its use, the maximum coeffi cient of multiplication (CM) for both varieties (9,0 ± 0,3 for the variety Dobryaya and 9,7 ± 0,4 for the variety Aquarel`) was obtained. The specifi city of varieties at action of low and high concentrations of BAP is revealed. It was noted, that a decrease in the nitrogen content in the medium reduces the CM in 1,5 time for the variety Dobryaya and negatively affects the length of regenerated plants of both varieties. An increase of the concentration of ferrum in 2 time in the medium did not affect on the CM and the sucrose substitution for glucose signifi cantly (in 2 times) increased CM. The positive effect of BAP and glucose on the micro-sprouts growth in length during cultivation is shown.