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Preliminary data of the Rcg1 gene determination in the grape varieties using a DNA marker

https://doi.org/10.18454/VSTISP.2017.6.8423

Abstract

The aim of the work was the approbation of the DNA marker UDV015 on genotypes of grapes - potential donors of the Rcg1 gene. DNA samples of seven wild forms of V. amurensis and fi ve grape varieties (interspecifi c hybrids) were analyzed by PCR. The reaction products were evaluated by capillary electrophoresis on an automated Genetic Analyzer ABI Prism 3130 and using GenMapper 4.1 program. According to preliminary data, four of the twelve analyzed genotypes may have Rcg1 gene allele determining resistance to the development of tumor formation of crown gall of grape.

About the Authors

E. T. Ilnitskaya
FSBSI «North-Caucasian Federal scientifi c center of horticulture, viticulture and winemaking»
Russian Federation


S. V. Tokmakov
FSBSI «North-Caucasian Federal scientifi c center of horticulture, viticulture and winemaking»
Russian Federation


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Review

For citations:


Ilnitskaya E.T., Tokmakov S.V. Preliminary data of the Rcg1 gene determination in the grape varieties using a DNA marker. Horticulture and viticulture. 2017;(6):9-12. (In Russ.) https://doi.org/10.18454/VSTISP.2017.6.8423

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ISSN 0235-2591 (Print)
ISSN 2618-9003 (Online)