Regenerative ability of Rubus L. genera explants of different origination

Abstract

The problem of working out effective, reliable, reputable technique for regenerants obtaining in leaf discs and others complex explants is very actual and important for different fields of plant science such as plant breeding, virology, plant propagation, genetic and physiology. In our experiments in vitro regeneration of whole plants from the leaf discs, leaves and intermodal segments of blackberry (Rubus sp.), and red raspberry (Rubus idaeus) has been studied. It was shown that the intermodal segments provided the highest level of shoot regeneration in comparison with leaf discs and leaves culture. Anther culture was characterized by lowest rate of shoots regeneration. The rate of callus formation in isolated anther culture depended on the stage of pollen grain development. For increasing of regenerants output in leaf discs culture the optimization of nutrient media components as far as auxins and cytokinins concentration was carried out; it was also established that leaf discs explants precultivation on low level illumination provided increasing of rate regeneration. The same effect could be obtained by means of wounding of leaf blade. In general the rate of regeneration depended on species and varieties peculiarities, nutrient media composition, cultivation conditions. The worked out techniques can be used for genetic engineering and virology investigation, somaclonal selection, plant propagation including “artifi cial seeds” creation.