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Taxonomic structure of tissue endophytic bacterial microbiome in clonal apple rootstock grown on sod-podzolic soils with variant properties
https://doi.org/10.31676/0235-2591-2021-6-36-44
Abstract
The taxonomic structure of tissue endophytic bacterial microbiome was comparatively studied in microplants (undifferentiated explant callus tissues, passage 25) and 5-year clonal apple rootstocks 57-490 and 54-118 cultured from corresponding tissues (passage 1) on sod-podzolic soils with variant granulometry, chemical, physical and physicochemical properties. Proteobacteria (91.6 %) predominated in vitro tissues among other endophytic bacterial phyla in rootstock 57-490, while Proteobacteria (52.5 %) and Firmicutes (47.4 %) — in rootstock 54-118. The endophytic Firmicutes ratio vs. in vitro tissues decreases (0.7-2.0 %) in roots and more severely (0-0.2 %) in leaves. Endophytic Actinobacteriota are revealed in 11.7 % in roots of the study rootstock in heavy loam soil, whilst in medium loam their ratio drops to 2.74.1 % in roots and 0.1-0.2 % in leaves. The phylogenetic diversity indices estimation for main endophytic bacterial phyla in apple rootstock tissue recovers their essentially lower diversity and evenness in culture endosphere (Shannon index 0.42-1.00) vs. open soil roots (1.34-2.08). The leaves Shannon index is typically low (0.06-0.13) indicating poor diversity and evenness of the main endophytic bacterial phyla.
Keywords
endophytic bacteria,
taxonomic structure,
apple rootstock,
soil properties,
phylogenetic diversity indices
Supporting agencies: The work was carried out with the financial support of the Russian Foundation for Basic Research (RFBR) grant, project No. 20-016-00201\21
About the Authors
V. V. Bobkova
Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery
Russian Federation
Russian Federation
Researcher, Department of Agrochemistry and Soil Science
Moscow
S. N. Konovalov
Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery
Russian Federation
Russian Federation
Sergey N. Konovalov, PhD (Biol.), Leading Researcher, Head of the Department of Agrochemistry and Soil Science
4, Zagor’evskaya Str., Moscow, 115598
M. T. Upadyshev
Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery
Russian Federation
Russian Federation
Dr. Sci. (Agric.), Member Corresponding of Russian Academy of Sciences, Chief Researcher, Head of the Virology Laboratory
Moscow
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Review
For citations:
Bobkova V.V., Konovalov S.N., Upadyshev M.T. Taxonomic structure of tissue endophytic bacterial microbiome in clonal apple rootstock grown on sod-podzolic soils with variant properties. Horticulture and viticulture. 2021;(6):36-44. (In Russ.) https://doi.org/10.31676/0235-2591-2021-6-36-44
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