The aim of the work was the approbation of the DNA marker UDV015 on genotypes of grapes - potential donors of the Rcg1 gene. DNA samples of seven wild forms of V. amurensis and fi ve grape varieties (interspecifi c hybrids) were analyzed by PCR. The reaction products were evaluated by capillary electrophoresis on an automated Genetic Analyzer ABI Prism 3130 and using GenMapper 4.1 program. According to preliminary data, four of the twelve analyzed genotypes may have Rcg1 gene allele determining resistance to the development of tumor formation of crown gall of grape.

The purpose of breeding of apple trees in the low mountains of Altai is the systematic improvement of the assortment of culture in Siberia. The main method of breeding was the geographically remote intersocross crossings and seed sowing from free pollination. Modern varieties are the third and fourth generation of Malus baccata. As a result of a purposeful long-term work on the breeding of apple trees by the staff of the Department of mountain horticulture of Lisavenko Research Institute of Horticulture of Siberia in Gorno-Altaisk 34 varieties of apple were created, 14 of which were included in the State register of breeding achievements and were allowed to use in the 10th region (Western Siberia). Since 2007, the assortment has qualitatively replenished with the varieties with the summer period of consumption of the fruits (Tolunay, Shushenskoe), with the autumn maturity of the fruits (Altynai, Bayan, Poklon Shukshinu, Surkhurai), with the winter term of consumption (Gorny Sinap). These varieties are adaptive to the severe conditions of the winter period of Siberia and have a high regenerative capacity after damage during critical winter periods, early ripening, high yielding, with regular fruit bearing, resistant to scab, with an average fruit mass from 56 to 97 g and maximum to 170 g.

The aim of studies was to create varieties, combining high winter resistance, regular and high yield, good taste and long keeping of fruits, available for fresh consumption as well as for processing. The studies were conducted in conditions of the forest-steppe of the Altai Ob area. All fi eld observations were conducted according to the common methodologies. 14 pear varieties have been created in the Research Institute of Horticulture of Siberia, 7 of them were included into the State register of breeding achievements, admitted for use, including summer varieties, such as Lel, Sibiryachka, late-ripening Kuyumskaya, early-autumn Karatayevskaya, Kupava, Svarog, late-autumn Perun. The main result of studies on breeding of pear in the Altai region are expounded in the paper. The main directions of pear breeding are defi ned: winter-resistance, yield, high qualities of fruits keeping quality. Characteristic of 4 new pear varieties with high economical-valuable features was presented. The method of obtaining new varieties was the geographically and systematically remote hybridization. The varieties pass the State variety trials. The introduction of new varieties into production will signifi cantly improve the available assortment.

The estimation of grape varieties obtained from different regions have been carried out in conditions of foreststeppe of Altai Krai. Methods proposed by State Variety Testing Agency have been used. The collection includes 195 varieties both domestic and foreign origin. Within 20 years of investigation there were no damage of roots as well as annual and perennial sprouts. Up to 74.2 % of buds were died because of asphyxiation. Fourteen varieties have been selected for high winter hardiness. Bud breaking was observed at May 1-20. The mean period of fl owering was June 6-28, berries ripening was September 12-24. Leaf fall was not observed. The largest berries (3.9-9.5 g) and bunches (209-1500 g) were observed on 11-th varieties. Palatability of fresh berries was ranged from 3.5 to 9.8 points. Soluble solids in berries were ranged from 10.1 up to 23.8 %, sugar - 5.7-23.7 %, acidity - 0.3-2.3 %. The average yield was ranged from 2.7 to 8.0 kg/bush, up to maximum of 25.0 kg/bush. Signifi cant variation in productivity for most of varieties have been recorded (20.4-53.8 %). Statistically it was proved that the yield of grapes infl uenced by the factor the year is 41.7 % as well as by factor of variety origin is 30.0 %. Rest of factors have infl uence totally 28.3 %. Wasps were indicated as main pest. Resistance to mildew was shown by 132 varieties. Ripening of shoots was ranged from 26 to 93 %. As exception in 2013 at most of varieties shoots were ripened at low level (0-36.9 %). As a results of long period estimation it have been selected 31 varieties of grape distinguished by range of economically valuable features. Promising varieties are recommended for cultivation in climate condition of South part of Western Siberia.

The results of research of hybrids of the genus Lilium L. of the “Tango” group from Section I - Asian hybrids, created on the basis of domestic and foreign varieties in conditions of the forest-steppe area of Altai Territory are presented. Estimation of decorative features of 26 selective forms of lilies belong to “Tango” group, based on 100-point scale proposed by State varieties testing service was carried out, to select most adapted varieties to conditions of Western Siberia. The range of estimation varied from 75.2 (41/12-3) up to 90.0 points (16/12-2). According to the results of the estimation, 8 forms were singled out to the elite, who scored 90-87.3 points: seven were singled out to the average fl owering period and one (18 / 12-8) was singled out to late group. They have a different color of leaves of the perianth (white, pink, yellow, lemon, yellow-orange) and thickened fl eck (magenta, crimson and red). A comprehensive evaluation of elite forms was carried out according to the methodology of V. N. Bylov. The rating of elite forms regards economic and biological features is ranged from 20 up to 44 points and included following characteristics: fl owering period, fl ower size, fl owering productivity, reproductive capacity and plant height. Abundantly fl owering hybrids with a bloom period of more than 20 days (16/12-2, 41/12-4 and 22/12-1), as well as varieties of high reproductive capacity (18/12-1, 18/12-3 and 41/12-1) have been selected. In 2016 with large sire of fl ower (diameter from 14.5 to 16.0 cm) were fi ve elite forms (17/12-1, 16/12-2, 22/12-1, 41/12-1 and 18/12-3). Based on comprehensive assessment, most valuable elite forms have been proposed: 16/12-2 (134 points), 22/12-1 (128.3) and 18/12-1 (124 points).

Russian Research Institute of Floriculture and Subtropical Crops holds a several collections of subtropical and southern fruit crops. Jujube collection (Zizyphus jujuba Mill.) includes twenty accessions. The best large-fruiting varieties were identifi ed in our study: ‘Vakhsh’, ‘Ta-yang-tszao’, ‘U-sin-hun’, ‘Khurmai’, ‘Yuzhanin’, ‘Chinese 2A’. The varieties proned to parthenocarpic fruiting ‘Ta-yan-tszao’, ‘U-sin-hun’ were determined. The most productive varieties in the humid subtropics of Russia were - ‘Ta-yang-tszao’, ‘Yuzhanin’, ‘Burnim’, ‘Vakhsh’, ‘U-sin-hun’, ‘Hissar’, F-201. Fresh jujube fruits are very diverse in their taste, and table varieties have been established for fresh consumption with lined in size fruits, intensive color, juicy fl esh, sweet taste: ‘Wu-hsing-hoon’, ‘Tayang- tszao’, ‘Khurmai’, ‘Vakhsh’. Among them ‘Khurmai’ and ‘Vakhsh’ have soft almost imperceptible fruit skin. Fruits of ‘Yuzhanin’ and ‘Chinese 2A’ are juicy with a delight balance of acid and sugar at full maturity. Among universal varieties there are ‘Wu Xing-hoon’, ‘Da-bai-tszao’, ‘Yuzhanin’, ‘Vakhsh’, ‘Burnim’, ‘Khurmai’. Most of the varieties from the collection are suitable for canning and making dried and candied fruits. The jujube fruits, especially some varieties of medium and late maturation, are prone to cracking. The most resistant to cracking in the humid subtropics are ‘Chinese-2A’, ‘Hissar’, ‘F-201’ and small-fruited forms. Fruits of varieties ‘Khurmai’, ‘U-sin-hun’ crack in the range of 8-10 % in the stage of incomplete maturity. The most severe cracking of fruits was observed in the variety ‘Yuzhanin’. Some varieties and forms (‘Hissar’, F-201) are highly ornamental and can be widely used in landscape construction.

Winter hardiness and productivity estimation of 13 golden currant varieties and selected forms breeding in Lisavenko Research Institute of Horticulture of Siberia have been done in years 2011-2015. Evaluation of climate condition during years of investigation has been carried out. Level of climate infl uence on plants has been detected. It was established that all samples have suffi cient adaptability to extreme weather conditions of Altai. The overwintering of plants, including severe conditions in the autumn-winter period of 2012/13 was passed safely without freezing of branches and generative buds. Due to increased air temperatures in year 2014, the vegetation was started earlier compared to mean data. At the end of April, there was frost, that damaged part of the fl oral buds in the brush. As a result of estimation, some varieties like Levushka and Otrada as well as several hybrids resistant to freezing of fl ower buds by spring frosts have been selected. Flowering both of varieties and elite forms were abundant (4,0-5,0 points). The yield of estimated varieties was ranged from 2,0 up to 6,5 kg per bush (5,0-16,3 t/ha), weight of berries was from 0,6 to 3,8 g. The varieties Otrada, Levushka, Yubiley Altaya has been selected for high productivity (11,5; 10,8; 9,0 t/ha respectively). Some other varieties have been also selected for high productivity: 4197-06-1, 4189-06-9 3685-99-13 and 4190-06-13. Productivity of Ida and Otrada varieties as well as variety under selection 4190-06-4 is resistant regardless of weather conditions, the variation index is 3,4- 9,5%. The weight of berries depends on many factors, including climate conditions of the year. In years 2013 and 2014, the amount of precipitation during the vegetation period was higher compared to other years, that infl uenced in increasing of weight of berries up to 9,0-33,0 %.

The article presents the results of the research of the content of basic groups of bio-antioxidants in the fruits of Altai apple varieties: vitamin C, biofl avonoids, and pectin substances. The research was conducted in Lisavenko Research Institute of Horticulture for Siberia in 2006-2016. Material of the research includes ripe fruits of 11 Altai apple varieties of summer and autumn maturity, which are most promising from the economic and biochemical standpoint. Total content and component composition of phenolic compounds were determined in ethanolic extracts of fruits by spectrophotometric and colorimetric methods; total content of vitamin C was determined by indophenol titration with potentiometric indication; and total content of pectin substances was determined by titration. It was established that the content of bioactive compounds in the fruits of the concerned varieties is highly variable. Comparative analysis in this aspect was conducted. The content of vitamin C in the fruits of Altai apple varieties ranges from 4,8-31,8 mg/100 g, biofl avonoids - 264,9-621,0 mg/100 g, pectin - 0,5-2,9 %, pectin compounds - 1,4-4,3 % in fresh weight. Phenolic compound of fruits differs mainly in quantitative variations of specifi c fractions. The majority of crops are characterized by domination of proanthocyanidin (63 %), to a lesser extent by catechins (21 %), fl avonols (12 %) and anthocyanins (4 %). The varieties of apple differ not only in quantitative content of pectin compounds in fruits, but also in the degree of etherifi cation of pectin. Valuable varieties were revealed as sources of high content of bio-antioxidants for their future use in breeding and technological processing: on the content of vitamin C there were the varieties Altaiskoe Rumyanoe, Altaiskoe Yantarnoe, Zhebrovskoe, Yunga, Altaiskoe Bagryanoe, Souvenir Altaya; on the content of biofl avonoids there were the varieties Souvenir Altaya, Altaiskoe Bagryanoe, Zhebrovskoe, Smuglyanochka; on the content of pectin compounds there were the varieties Altaiskoe Yantarnoe, Altaiskoe Rumyanoe, Altaiskoe Zimnee, Smuglyanochka, Yunga, Altaiskoe Bagryanoe.

The aim of our study was to select the most suitable honeysuckle varieties among the new varieties of other Scientific institutions for cultivation in the forest-steppe of the Altai Territory. Among the 13 varieties on the collection plot the most large-fruited were the varieties of the institution “Bakcharskoye” breeding: “Vostorg”, “Strezhevchanka” and “Doch Velikana”. The mass of their fruit was at average from 1,2 to 2,0 g, the maximum was from 1,4 to 2,9 g, the fruit was a dessert taste. The varieties of N. I. Vavilov Research Institute of Plant Industry were with small and average mass of fruits, a dessert taste and fall of the fruit. It is possible to note the “Laura” variety as the largest (1,3-1,5 g). The variety “Pamyat Kuminova” breeding in I. V. Michurin research horticultural institute has fruits with the mass of 0,9-1,4 g and dessert taste, with a palpable aroma and a strong fall of the fruit. Among the varieties breeding in Kamchatka research agricultural institute the “Atlant” variety was distinguished by large fruits (0,9-1,6 g) of sweet-sour taste and without of fruit fall. According to preliminary results the introduced varieties are quite suitable for cultivation in orchards in the conditions of the forest-steppe of the Altai Territory.